Back to Tiffany Chase, and back to Shadowgirl, the previous episode. Chase is abducted by our Harley stand-in and her Robin goons. This time Shadowgirl is out of her heroine costume. She's chained up and whipped as the villainess calls the strikes like a catcher calls pitches in baseball.
Back to Gloria and back to Heroine Kombat. This time we have a Poison Ivy gimmick vs a Dark Supergirl. During the fight, the Nightshade character uses poisonous breath to gain the upper hand on Vega and then uses this advantage to give her a spanking, which Vega really gets a kick out of.
...and back to Tiffany Chase . Earlier in the Your Soul is Mine series, the first episode features the encounter between Trinity (Chase) and The Black Queen (Hess) which sets up who our main protagonist and villain of the series are. The Black Queen gets the upper hand on Trinity, making her temporarily unable to defend herself, and so the Queen uses her whip to assert dominance over her disadvantaged opponent.
Veering off to Kristina Hess, this is from another HK video, and co-stars Mario Rocha. Kristina is a Wonder Woman knockoff named Talon. There's a quick 1-smack at the beginning of the clip, then Kristina gets spanked by Rocha, just before she meets her ultimate fate, towards the end of this episode.
Staying in Heroine Kombat and back to Tiffany Chase. This time she's paired up with the lady and character who started the thread (Lyndsay Lamb) and during this sequence, Lyndsay's Tsuki is using Shadowgirl's own cape against her. First choking Shadowgirl with it and then as she tries to crawl away from the attack, Lyndsay uses the cape to whip Chase's backside. After a bit of back and forth with the cape attacks, Shadowgirl finds herself back in the same position for one last strike.
... Back to Tiffany Chase. In the second "canon" Shadowgirl series, our Harley knock-off has been switched out for a different blonde lady in a catsuit, but the weird Robin goons remain. This time 2 of the goons stretch out Shadowgirl, while another goon delivers backhanded smacks to her bottom. There's also a bonus aoh whipping scene, delivered by Gretchen (Domina, aka lady in the catsuit).
Going back to 2012, which I think was the start-up year for HL. So, I haven't checked 100%, but I think this is the first spanking to appear in one of their videos.